UW-Madison Dept of Bacteriology
Pictures of RLG Richard L. Gourse

Professor of Bacteriology
Chair, Department of Bacteriology

5470 Microbial Sciences Building
1550 Linden Drive

Office: (608) 262-9813
Lab: (608) 262-2419
email icon for Goursergourse@bact.wisc.edu

BioPublicationsLab Personnel
Education
A.B., American Civilization, Brown University 1971
M.A.T., Education, Brown University 1973
Ph.D., Cell and Molecular Biology, Brown University 1980
Postdoctoral Research: Brown University; University of Wisconsin-Madison
Start and Promotion Dates
  • Assistant Professor: 1988
  • Associate Professor: 1991
  • Full Professor: 1996
Research Focus

We study fundamental issues in the molecular biology of gene expression in bacteria using cutting edge biochemical and genetic techniques. At present, we are focused primarily on the mechanism of transcription initiation, the architecture of transcription complexes, the relationship between structure and function of RNA polymerase (RNAP), and the mechanisms by which the transcription apparatus responds to signals from inside and outside of the cell. We have studied the control of ribosome synthesis in Escherichia coli, because this is so central to cell function, because rRNA promoters account for the majority of transcription in the cell, and because the genes for rRNA and other parts of the translation machinery display a fascinating and complex network of regulatory responses. In recent years we have discovered that the factors responsible for controlling rRNA synthesis also regulate hundreds of other genes, and we have begun to study the regulation of many of those genes as well. Current projects in the lab include studies on (i) the conformational changes in the promoter-RNAP complex that account for the tremendous variation in activity that occurs in response to differences in natural promoter sequences, (ii) the transcription factors ppGpp and DksA and their interactions with RNAP responsible for transcription regulation, and (iii) a transcription factor that facilitates RNA polymerase assembly. In addition, using tools of cell biology including fluorescence microscopy, we have discovered that bacteria contain nucleolus-like structures; i.e. rRNA operons come together in space even though the rRNA genes are spread out over 180 degrees of the circular E. coli chromosome. We are now exploring the factors that are responsible for these long-range interactions and their contributions to chromosome folding.

Awards
  • 2007 NIH Merit Award
  • 2003 American Academy of Microbiology Fellow
  • 2003 American Association for the Advancement of Science (AAAS) Fellow
Teaching
  • Microbiology 612: Prokaryotic Molecular Biology
Activities
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Department of Bacteriology
University of Wisconsin-Madison
Microbial Sciences Building
1550 Linden Dr. Madison, WI 53706

Phone (608) 262-2914
Department Chair: Richard Gourse
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