UW-Madison Dept of Bacteriology

Select Publications

1. Lewis, J. L., L. Stamper, and J. C. Escalante-Semerena. 2009. Regulation of expression of the tricarballylate utilization operon (tcuABC) of Salmonella enterica by TcuR and Crp. Res. Microbiol. 160:179-186. PMID: 19284970.

1. Gardner, J. G., and J. C. Escalante-Semerena. 2009. In Bacillus subtilis, functions encoded by the acuABC operon are involved in the sirtuin-dependent control of acetyl-CoA synthetase activity. J. Bacteriol. 191:1749-1755. PMID: 19136592.
   
   Lewis, J. A., J. M. Boyd, D. M. Downs, and J. C. Escalante-Semerena. 2009. Involvement of the global regulatory protein Cra in the expression of the iscRSUA operon revealed during studies of tricarballylate catabolism in Salmonella enterica. J. Bacteriol. 191:2069-2076. PMID: 19136587
   
   Mera, P. E., St Maurice, M., I. Rayment, and J. C. Escalante-Semerena. 2009. Residue Phe112 of the human-type corrinoid adenosyltransferase (PduO) enzyme of Lactobacillus reuteri is critical to the formation of the 4-coordinate Co(II) corrinoid substrate and to the activity of the enzyme. Biochemistry 48:3138-3145. PMID: 19236001.
   
   Otte, M., and J. C. Escalante-Semerena. 2009. Biochemical characterization of the GTP:Adenosylcobinamide-phosphate guanylyltransferase (CobY) enzyme of the thermophilic archaeon Methanocaldococcus jannaschii. Biochemistry 48:5882-5889. PMID: 19489548.
   
   Gray, M. J., and J. C. Escalante-Semerena. 2009. In vivo analysis of cobinamide salvaging in Rhodobacter sphaeroides 2.4.1. J. Bacteriol. 191:3842-3851. PMID: 19376876.

Escalante-Semerena, J. C., and M. J. Warren.  5 August 2008, posting date. Chapter 3.6.3.8, Biosynthesis and Use of Cobalamin (B12). In A. Böck, R. Curtiss III, J. B. Kaper, P. D. Karp, F. C. Neidhardt, T. Nyström, J. M. Slauch, and C. L. Squires (ed.), EcoSal—Escherichia coli and Salmonella: cellular and molecular biology. ASM Press, Washington, D.C.

St. Maurice, M, P. E. Mera, K. Park, T. C. Brunold, J. C. Escalante-Semerena, and I. Rayment. 2008. Structural characterization of a human-type corrinoid adenosyltransferase confirms that coenzyme B12, is synthesized through a four-coordinate intermediate. Biochemistry 47:5755-5766. PMID: 18452306.

Park, K., P. E. Mera, J. C. Escalante-Semerena, and T. C. Brunold. 2008. Kinetic and spectroscopic studies of the ATP:C(I)rrinoid adenosyltransferase PduO enzyme of Lactobacillus reuteri. Biochemistry 47:9007-9015. PMID: 18672897.

Gray, M. J., N. K. Tavares, and J. C. Escalante-Semerena. 2008. The genome of Rhodobacter sphaeroides strain 2.4.1 encodes functional cobinamide salvaging systems of archaeal and bacterial origins. Mol. Microbiol. 70:824-836. PMID: 18808385.

 Gardner, J. G., and J. C. Escalante-Semerena. 2008. Biochemical and mutational analyses of AcuA, the acetyltransferase enzyme that controls the activity of the acetyl-Coenzyme A synthetase (AcsA) in Bacillus subtillis. J. Bacteriol. 190:5132-5136.

Rocco, C. J., K. L. Dennison, V. A. Klenchin, I. Rayment, and J. C. Escalante-Semerena. 2008. Construction and use of new cloning vectors for the rapid isolation of recombinant proteins from Escherichia coli. Plasmid 59:231-237

Ruiz-Sanchez, P., S. Mundwiler, B. Spingler, N. R. Buan, J. C. Escalante-Semerena, and R. Alberto. 2008. Syntheses and characterization of vitamin B12-Pt(II) conjugates and their adenosylation in an enzymic assay. J. Biol. Inorg. Chem.13:335-347

St Maurice, M., P. Mera, M. P. Taranto, F. Sesma, J. C. Escalante-Semerena, I. Rayment. 2007. Structural characterization of the active site of the PduO-type ATP:Co(I)rrinoid adenosyltransferase from Lactobacillus reuteri. J. Biol. Chem. 282:2596-2605.

Gray, M. J., and J. C. Escalante-Semerena. 2007. Single-enzyme conversion of FMNH2 to 5,6-dimentylbenzimidazole, the lower ligand of B12. Proc. Natl. Acad. Sci. USA 104:2921-2926.

Zayas, C. L., and J. C. Escalante-Semerena. 2007. Reassessment of the late steps of coenzyme B12 synthesis in Salmonella enterica: Evidence that dephosphorylation of adenosylcobalamin-5’-phosphate by the CobC phosphatase is the last step of the pathway. J. Bacteriol. 189:2210:2218.

Brinsmade, S. R., and J. C. Escalante-Semerena. 2007. In vivo and in vitro analyses of single-amino acid variants of the Salmonella enterica phosphotransacetylase (Pta) enzyme provide insights into the function of its N-terminal domain. J. Biol. Chem. 282:2596-2605.

Garvey, G. S., C. J. Rocco, J. C. Escalante-Semerena, and I. Rayment. 2007. The three-dimensional crystal structure of the PrpF protein of Shewanella oneidensis. Insights into its biological function. Protein Sci. 16:1274-1284.

Escalante-Semerena, J. C. 2007. Conversion of cobinamide into adenosylcobamide in bacteria and archaea. J. Bacteriol. 189:4555-4560.

Lewis, J. A., and J. C. Escalante-Semerena. 2007. Tricarballylate utilization in Salmonella enterica: The TcuB protein uses 4Fe-4S centers and heme to transfer electrons from FADH2 in the tricarballylate dehydrogenase (TcuA) enzyme to electron acceptors in the cell membrane. Biochemistry 46:9107-9115.

Otte, M. J. D. Woodson, and J. C. Escalante-Semerena. 2007. The thiamine kinase (YcfN) enzyme plays a minor but significant role in cobinamide salvaging in Salmonella enterica. J. Bacteriol. 189:7310-7315.

Garrity, J., J. G. Gardner, W. Hawse, C. Wolberger, J. C. Escalante-Semerena. 2007. N-Lysine propionylation controls the activity of propionyl-CoA synthetase. J. Biol. Chem. 282:30239-30245.

Zayas, C. L., K. R. Claas, and J. C. Escalante-Semerena. 2007. The CbiB protein of Salmonella enterica is an integral membrane protein involved in the last step of the de novo corrin ring biosynthetic pathway. J. Bacteriol. 189:7697-7708.

Mera, P. E.,M. St Maurice, I. Rayment, J. C. Escalante-Semerena. 2007. Structural and functional analyses of the human-type corrinoid adenosyltransferase (PduO) from Lactobacillus reuteri. Biochemistry 46:13829-13836.

Escalante-Semerena, J. C., and M. J. Warren. 2007. Biosynthesis and Use of Cobalamin (B12). EcoSal American Society for Microbiology.

Gardner, J., F. Grundy, T. M. Henkin, and J. C. Escalante-Semerena. 2006. Control of acetyl-CoA synthetase (AcsA) activity by acetylation/aeacetylation without NAD+ involvement in Bacillus subtilis. J. Bacteriol. 188:5460-5468.

Lewis, J. A., and J. Escalante-Semerena. 2006. The FAD-dependent tricarballylate dehydrogenase (TcuA) enzyme of Salmonella enterica converts tricarballylate into cis-aconitate. J. Bacteriol. 188:5479-5486.

Kim, Y. R., S. R. Brinsmade, Z. Yang, J. Escalante-Semerena, and J. Fierer. 2006. Mutation of phosphotransacetylase but not isocitrate lyase reduces the virulence of Salmonella enterica serovar Typhimurium in mice. Infect & Immun 74:2498-2502.

Zayas, C. L., J. D. Woodson, and J. C. Escalante-Semerena. 2006. The cobZ gene of Methanosarcina mazei Gö1 encodes the nonorthologous replacement of the alpha-ribazole-5'-phosphate phosphatase (CobC) enzyme of Salmonella enterica. J. Bacteriol. 188:2740-2743.

Buan, N. R., and J. C. Escalante-Semerena.2006. Studies of the CobA-type ATP:Co(I)rrinoid adenosyltransferase enzyme of Methanosarcina mazei strain Gö1. J. Bacteriol. 188:3543-3550.

Buan, N. R., and J. C. Escalante-Semerena.2006. Purification and initial characterization of the ATP:Cob(I)alamin adenosyltransferase (EutT) enzyme of Salmonella enterica. J. Biol. Chem. 281:16971-16977.

Woodson, J. D., and J. C. Escalante-Semerena.2006. The cbiS gene of the archaeon Methanopyrus kandleri AV19 encodes a bifunctional enzyme with adenosylcobinamide amidohydrolase and alpha-ribazole-phosphate phosphatase activities. J. Bacteriol. 188:4227-4235.

Starai, V. J., J. G. Gardner, and J. C. Escalante-Semerena. 2005. Residue Leu641 of acetyl-CoA synthetase (Acs) is critical for the acetylation of residue Lys609 by the protein acetyltransferase (Pat) enzyme of Salmonella enterica. J. Biol. Chem. 280:26200-26205.

Stich, T. A., N. R. Buan, J. C. Escalante-Semerena, and T. C. Brunold. 2005. Spectroscopic and computational studies of the ATP:corrinoid adenosyltransferase (CobA) from Salmonella enterica: insights into the mechanism of adenosylcobalamin biosynthesis. J. Amer. Chem. Soc. 127:8710-8719.

Buan, N. R., and J. C. Escalante-Semerena. 2005. Computer-assisted docking of flavodoxin with the ATP:co(I)rrinoid adenosyltransferase (CobA) enzyme reveals residues critical for protein:protein interactions but not for catalysis. J. Biol. Chem. 280:40948-40956.

Woodson, J. D., A. Reynolds, and J. C. Escalante-Semerena. 2005. ABC transporter for corrinoids in Halobacterium sp. strain NRC-1. J. Bacteriol. 187:5901-5909.

Starai, V. J., J. Garrity, and J. C. Escalante-Semerena. 2005. Acetate excretion during ethanolamine catabolism by Salmonella enterica requires phosphotransacetylase (EutD) activity and acetate recapture requires acetyl-CoA synthetase activity. Microbiology 151:3793-3801.

Brinsmade, S. R., T. Paldon, and J. C. Escalante-Semerena. 2005. Minimal functions and physiological conditions required for growth of Salmonella enterica on ethanolamine in the absence of the metabolosome. J. Bacteriol. 187:8039-8046.

Grimek, T. A., and J. C. Escalante-Semerena. 2004. The acnD gene of Shewenella oneidensis and Vibrio cholerae encodes a new Fe/S-dependent 2-methylcitrate dehydratase enzyme that requires prpF function in vivo. J. Bacteriol. 186:454-462.

Woodson, J. D., and J. C. Escalante-Semerena. 2004. CbiZ, a new enzyme for cobamide biosynthesis in archaea, has amidohydrolase activity that converts adenosylcobinamide to adenosylcobyric acid. Proc. Natl. Acad. Sci. USA 101:3195-3196.

Brinsmade, S. R., and J. C. Escalante-Semerena. 2004. The eutD gene of Salmonella enterica encodes a protein with phosphotransacetylase enzyme activity. J. Bacteriol. 186:1890-1892.

Lewis, J. A., A. R. Horswill, B. Schwem, and J. C. Escalante-Semerena. 2004. The tricarballylate utilization (tcuRABC) genes of Salmonella enterica serovar Typhimurium LT2. J. Bacteriol. 186:1629-1637.

Maggio-Hall, L. A., K. Claas, and J. C. Escalante-Semerena. 2004. The last step in coenzyme B12 synthesis is localized to the cell membrane in bacteria and archaea. Microbiology 150:1385-1395.

Starai, V. J., H. Takahashi, J. D. Boeke, and J. C. Escalante-Semerena. 2004. A link between transcription and intermediary metabolism: a role for Sir2 in the control of acetyl-CoA synthase. Curr. Op. Microbiol. 7:1-5.

Starai, V. J., and J. C. Escalante-Semerena. 2004. Acetyl-Coenzyme A Synthetase (AMP-forming). Cell. Molec. Life Sci. 61:2020-2030.

Starai, V. J., and J. C. Escalante-Semerena. 2004. Identification of the protein acetyltransferase (Pat) enzyme that acetylates acetyl-CoA synthetase in Salmonella enterica. J. Mol. Biol. 340:1005-1012.

Woodson, J. D., R. F. Peck, M. P. Krebs, and J. C. Escalante-Semerena. 2003. The NTP:Adenosylcobinamide-phosphate nucleotidyltransferase enzyme encoded by the cobY gene of the archaeon Halobacterium sp. NRC-1 is required for de novo cobamide synthesis. J. Bacteriol. 185:311-316.

Gulick, A. M., V. J. Starai, A. R. Horswill, K. M. Homick, and J. C. Escalante-Semerena. 2003. The 1.75Å crystal structure of acetyl-CoA synthetase bound to adenosine-5'-propylphosphate and coenzyme A. Biochemistry 42:2866-2873.

Palacios, S, V. J. Starai, and J. C. Escalante-Semerena. 2003. Propionyl-coenzymeA is a common intermediate in the 1,2-propanediol and propionate catabolic pathways needed for the expression of the prpBCDE operon during growth of Salmonella enterica on 1,2-propanediol. J. Bacteriol 185:2802-2810.

Maggio-Hall, L. A., and J. C. Escalante-Semerena. 2003. Alpha-5,6-Dimethylbenzimidazole, adenine dinucleotide (alpha-DAD), a putative new intermediate in coenzyme B12 biosynthesis. Microbiology149:983-990.

Starai, V. J., H. Takahashi, J. D. Boeke, and J. C. Escalante-Semerena. 2003. Short-chain fatty acid activation by acyl-coenzyme A synthetases requires SIR2 function in Salmonella enterica and Saccharomyces cerevisiae. Genetics 163:545-555

Starai, V. J., I. Celic, R. N. Cole, J. D. Boeke, and J. C. Escalante-Semerena. 2002. Sir2 controls acetyl-coenzyme A synthetase by deacetylation of an active site lysine residue. Science 298:2390-2392.

Gulick, A. M., A. R. Horswill, Thoden, J. B., J. C. Escalante-Semerena, and I. Rayment. 2002. Pentaerythritol propoxylate: A new crystallization agent and cryoprotectant induces crystal growth of 2-methylcitrate dehydratase. Acta Crystal.D58:306-309.

Warren, M. J., E. Raux, H. Schubert, and J. C. Escalante-Semerena. 2002. The biosynthesis of adenosylcobalamin (vitamin B12).Nat. Prod. Rep. 19:390-412.

Cheong, C.-G., C. B. Bauer, K. R. Brushaber, J. C. Escalante-Semerena, and I. Rayment.2002. The three-dimensional structure of L-threonine-O-3-phosphate decarboxylase (CobD) enzyme from Salmonella enterica. Biochemistry 41:4798-4808.

Horswill, A. R., and J. C. Escalante-Semerena. 2002. Characterization of the propionyl-coenzyme A synthetase (PrpE) enzyme of Salmonella enterica: Essential role of the Lys592 residue in propionyl-AMP synthesis. Biochemistry 41:2379-2387.

Fonseca, M. V., N. R. Buan, A. R. Horswill, I. Rayment, and J. C. Escalante-Semerena. 2002. The ATP:Co(I)rrinoid adenosyltransferase (CobA) enzyme of Salmonella enterica requires the 2*-OH group of ATP for function and yields inorganic triphosphate as by-product. J. Biol. Chem. 277:33127-33131.

Cheong, C.-G., J. C. Escalante-Semerena, and I. Rayment. 2002. Structural studies of the L-threonine-O-3-phosphate decarboxylase (CobD) enzyme from Salmonella enterica: The apo, substrate, and product-aldimine complexes. Biochemistry 41:4798-4808.

Cheong, C.-G., J. C. Escalante-Semerena, and I. Rayment. 2002. Capture of a labile substrate by expulsion of water molecules from the active site of nicotinate mononucleotide:5,6-dimethylbenzimidazole phosphoribosyltransferase (CobT) from Salmonella enterica. J. Biol. Chem. 277:41120-41127.

Horswill, A. R., and J. C. Escalante-Semerena. 2001. In vitro conversion of propionate to pyruvate by Salmonella enterica enzymes: 2-Methylcitrate dehydratase (PrpD) and aconitase enzymkes catalyze the conversion of 2-methylcitrate to 2-methylisocitrate. Biochemistry 40:4703-4713.

Horswill, A. R., A. R. Dudding, and J. C. Escalante-Semerena. 2001.Studies of propionate toxicity in Salmonella enterica serovar Typhimurium LT2 identify 2-methylcitrate as a potent inhibitor of cell growth. J. Biol. Chem. 276:19094-19101.

Bauer, C., M. V. Fonseca, H. M. Holden, J. B. Thoden, T. B. Thompson, J. C. Escalante-Semerena, and I. Rayment. 2001. The three-dimensional structure of ATP:corrinoid adenolsyltransferase of Salmonella typhimurium (CobA) in its free state, complexed with MgATP, and a ternary complex of CobA, hydroxocobalamin and MgATP. Biochemistry 40:361-374.

Fonseca, M. V., and J. C. Escalante-Semerena. 2001. An in vitro reducing system for the conversion of cob(III)alamin to adenosylcobalamin. J. Biol. Chem. 276:32101-32108.

Chowdhury, S., M. G. Thomas, J. C. Escalante-Semerena, and R. Banerjee. 2001. The coenzyme B12 analog 5'-deoxyadenosylcobinamide-GDP supports catalysis by methylmalonyl-CoA mutase in the absence of trans ligand coordination. J. Biol. Chem. 276:1015-1019.

Cheong, C.-G., J. C. Escalante-Semerena, and I. Rayment. 2001. Structural investigation of the biosynthesis of alternative lower ligands for cobamides by nicotinate mononucleotide:5,6-dimethylbenzimidazole phosphoribosyltransferase (CobT) from Salmonella enterica. J. Biol. Chem. 276:37612-37620.

Smith, J. S., C. B. Brachmann, I. Celic, M. A. Kenna, S. Muhammad, V. Starai, J. Avalos, J. C. Escalante-Semerena, C. Grubmeyer, C. Wolberger, J. D. Boeke. 2000. A phylogenetically conserved NAD-dependent protein deacetylase in the Sir2 protein family. Proc. Natl. Acad. Sci. USA 97:6658-6663.

Palacios, S., and J. C. Escalante-Semerena. 2000. prpR, ntrA, and ihf functions are required for expression of the prpBCDE operon encoding enzymes that catabolize propionate in Salmonella typhimurium LT2. J. Bacteriol. 182:182:905-910.

Fonseca, M., and J. C. Escalante-Semerena. 2000. Reduction of cob(III)alamin to cob(II)alamin in Salmonella enterica serovar Typhimurium LT2. J. Bacteriol.182:4304-4309.

Thomas, M. G., and J. C. Escalante-Semerena. 2000. Identification of an alternative NTP:5'-deoxyadenosylcobinamide phosphate nucleotidyltransferase in Methanobacterium thermoautotrophicum deltaH. J. Bacteriol. 182:4227-4233.

Downs, D. M., and J. C. Escalante-Semerena. 2000. Impact of genomics and genetics on the elucidation of microbial metabolism. Methods 20:47-54.

Thomas, M. G., T. Thompson, I. Rayment, and J. C. Escalante-Semerena. 2000. Analysis of the kinase activity of the adenosylcobinamide kinase/adenosylcobinamide-phosphate guanylyltransferase (CobU) adenosylcobalamin biosynthetic enzyme of Salmonella typhimurium J. Biol. Chem. 275:27576-27586.

Maggio-Hall, L. A., and J. C. Escalante-Semerena. 1999. In vitro synthesis of the nucleotide loop of cobalamin by Salmonella typhimurium enzymes. Proc. Natl. Acad. Sci. USA 96:11798-11803.